The crash happened on the M90 southbound at junction 2A, by Dunfermline, around 2.30pm on Tuesday.
Pictures from the scene, shared on the Fife Jammer Locations Facebook page, show all three vehicles in the bus lane with one of the cars visibly damaged and debris strewn across one lane of the road.
It is not yet know if there were any injuries.
Lengthy tailbacks on the motorway were also reported following the crash on Tuesday.
A Police Scotland spokesperson said: “We received a report of a three-vehicle crash on the M90 southbound at Junction 2A, Dunfermline, around 2.30pm on Tuesday, 12 October, 2021. Officers remain at the scene.”
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